Zespół naukowców z Japonii i Tajwanu udowadnia w artykule Degradative Effect of Nattokinase on Spike Protein of SARS-CoV-2, że enzym nattokinaza jest zdolny do rozpuszczania kolca białka w organizmie człowieka.
https://www.mdpi.com/1420-3049/27/17/5405 Abstract The coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), emerged as a pandemic and has inflicted enormous damage on the lives of the people and economy of many countries worldwide. However, therapeutic agents against SARS-CoV-2 remain unclear. SARS-CoV-2 has a spike protein (S protein), and cleavage of the S protein is essential for viral entry. Nattokinase is produced by Bacillus subtilis var. natto and is beneficial to human health. In this study, we examined the effect of nattokinase on the S protein of SARS-CoV-2. When cell lysates transfected with S protein were incubated with nattokinase, the S protein was degraded in a dose- and time-dependent manner. Immunofluorescence analysis showed that S protein on the cell surface was degraded when nattokinase was added to the culture medium. Thus, our findings suggest that nattokinase exhibits potential for the inhibition of SARS-CoV-2 infection via S protein degradation.
4. Conclusions In this study, we demonstrated that nattokinase, a serine protease, degrades the S protein of SARS-CoV-2. To investigate whether nattokinase contained in natto extract could inhibit SARS-CoV-2 infection, we analyzed S protein degradation by mixing the S protein expression cell lysate and nattokinase in a dose- and time-dependent manner. The RBD of the S protein binds to the membrane-distal portion of the ACE2 protein. Natto extract has been reported to inhibit SARS-CoV-2 infection in Vero E6 cells via RBD degradation [16]. We demonstrated that S protein degradation by nattokinase was blocked by heat or protein-inhibitor treatments. Our data suggest that the protease activity of nattokinase plays a crucial role in S protein degradation. Taken together, these findings support the notion that the inhibition of SARS-CoV-2 infection by natto extract was due to S protein degradation by nattokinase. Thus, our data indicated that nattokinase and natto extracts have potential effects on the inhibition of SARS-CoV-2 host cell entry via S protein degradation.
Komentarz: Może zamiast czekać na nagłą śmierć warto zacząć kurować się natto, by pozbyć się z toksyny zwanej białkiem kolca z organizmu? Co wy na to drodzy zaszprycowani? "DLACZEGO NATTO? Pod względem właściwości zdrowotnych natto bije na głowę inne produkty sojowe i plasuje się na szczycie żywności funkcjonalnej, poddanej fermentacji. Ten jeszcze mało w Polsce znany japoński produkt wspiera ogólne zdrowie organizmu. A wszystko to dzięki czterem różnym składnikom: witaminie K2mk7, enzymowi zwanemu nattokinazą, izoflawonom sojowym oraz bakteriom probiotycznym (Bacillus Subtilis Natto). Nattokinaza upłynnia krew jest bezpieczniejszym i silniejszym środkiem niż aspiryna a dodatkowo rozpuszcza skrzepy. Zmniejsza poziom cholesterolu, w wyniku czego obniża zbyt wysokie ciśnienie krwi, nie dopuszczając do związanych z tym poważnych chorób."
